Journal: Journal of Lipid Research

Article Title: Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring [S]

doi: 10.1194/jlr.D061432

Figure Lengend Snippet: Kinetic models describing the in vivo metabolism of the HDL apolipoproteins on each HDL size fraction. SAAM II models highlighting the synthesis and removal pathways for each apolipoprotein across the HDL size fractions: ApoA-I (A), ApoA-II (B), ApoE (C), ApoM (D), ApoC-III (E) and ApoA-IV (F). Each model contains an input compartment, which represent the plasma amino acid precursor pool (D3-Leu tracer enrichment in plasma) expressed as a forcing function. The same D3-Leu tracer enrichment curve was used for all protein models per participant. The source compartment accounts for the time necessary for labeled protein to appear on each HDL size fraction in plasma. Source pathways: number next to arrow represents average flux (mg/kg/d) through each pathway for the three participants. Numbers in parentheses represent average percent source of each protein into each HDL size fraction. Removal pathways: numbers next to arrows are average rates (pools/day), and numbers in parentheses are average percent of protein removed by that pathway. EVD may represent a processing compartment that includes apoA-I on prebeta that has been secreted but is outside the systemic circulation. LA1 prebeta may be generated from α3 HDL through the release of LA1. ApoA-II α3 and α2 arrows: top dashed arrow is a source and bottom solid arrow is a removal pathway.

Article Snippet: Multicompartmental modeling Kinetic modeling was performed using SAAM II modeling software (SAAM Institute, Seattle, WA).

Techniques: In Vivo, Clinical Proteomics, Labeling, Generated